morphological and molecular characterization of cattle hydatid cysts in khorramabad, iran
نویسندگان
چکیده
background: hydatidosis is one of the zoonotic diseases which affect animals and human beings at the larval stage of echinococcusgranulosus, thereby playing a role in exacerbating the economic and health problems in iran. objectives: this study was conducted to investigate the strains of echinococcusgranulosus isolated from cattle hydatid cysts, in khorramabad, in the lorestan province, west of iran. methods: twenty six isolates of hydatid cyst of cattle from lung (24 samples) and liver (2 sample) organs were collected from golshan slaughterhouse, in khorramabad. all of the samples were transferred to the laboratory for morphometric characterization and molecular study. in morphological characterization, blade length of large (lbl) and small (sbl) hook and the ratio of blade length to total length in large (lbl/ltl) and small (sbl/stl) hooks and total length of large (ltl) and small (stl) hooks were measured. in molecular study, a partial sequence of cytochrome oxidase 1 (cox1) with 440 bp in length was amplified applying primers j3 and j4.5. genomic dna sequencing was performed by sanger’s method. results: the morphological results showed that there is no significant difference between isolated from cattle hydatid cyst and sensu stricto strain (p
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عنوان ژورنال:
تحقیقات دامپزشکیجلد ۷۱، شماره ۴، صفحات ۳۸۱-۳۸۷
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